Description
Kisspeptin (also known as metastin) is a naturally occurring neuroendocrine peptide that plays a pivotal role in human reproduction by stimulating gonadotropin-releasing hormone (GnRH) secretion. This small peptide binds to the GPR54 receptor in the hypothalamus, triggering pulsatile GnRH release and downstream secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Originally identified as a metastasis-suppressor gene product, kisspeptin has become a major focus in reproductive endocrinology. This educational protocol presents a once-daily subcutaneous approach for research use.
- Reconstitute: Add 3.0 mL bacteriostatic water → ~3.33 mg/mL concentration.
- Typical daily range: 100–200 mcg once daily (gradual titration).
- Easy measuring: At 3.33 mg/mL, 1 unit = 0.01 mL ≈ 33.3 mcg on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 100 mcg | 6 units (0.03 mL) |
| Weeks 3–8 (or 3–12) | 200 mcg | 12 units (0.06 mL) |
Frequency: Inject once daily subcutaneously. This schedule follows a conservative titration to assess individual response[4][5]. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
