Description
Epitalon (also known as Epithalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) developed from the naturally occurring pineal peptide epithalamin. Research indicates it may support telomerase activation, circadian rhythm regulation, and anti-aging pathways. Clinical studies employ short intermittent cycles (10–20 days) repeated 1–2 times annually], though extended titration protocols are also explored for research purposes.
- Reconstitute: Add 2.0 mL bacteriostatic water → 5 mg/mL concentration (10 mg total).
- Typical daily range: 5–10 mg once daily (subcutaneous injection).
- Easy measuring: At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg on a U-100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); reconstituted: refrigerate at 2–8 °C (35.6–46.4 °F); avoid freeze–thaw cycles.
Dosing & Reconstitution Guide
Educational guide for reconstitution and daily dosing
Standard Cyclic Protocol (2 mL = 5 mg/mL)
Literature-Based Approach: Clinical studies typically use 10–20 consecutive days of daily injections at 5–10 mg/day, followed by a break of several months (6–12 months), then repeat the cycle 1–2 times per year[4][5]. This intermittent approach appears optimal for maintaining biological benefits without continuous exposure.
| Cycle Day | Daily Dose (mg) | Units (per injection) (mL) |
|---|---|---|
| Days 1–5 | 5 mg (5000 mcg) | 100 units (1.00 mL) |
| Days 6–10 | 5 mg (5000 mcg) | 100 units (1.00 mL) |
| Days 11–20 (optional extension) | 5–10 mg (5000–10000 mcg) | 100–200 units (1.00–2.00 mL)* |
*Note: Doses above 5 mg require drawing from multiple reconstituted vials or splitting into two injections. For 10 mg dosing, consider reconstituting two vials and drawing 100 units from each.
Frequency: Inject once daily subcutaneously during the active cycle. After completing 10–20 days, take a break of 6–12 months before repeating.
Extended Titration Protocol (Optional for Research)
Some research protocols explore gradual dose escalation over 16 weeks to observe tolerance and effects. This approach starts at low doses and increases incrementally:
| Week | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Week 1 | 500 mcg | 10 units (0.10 mL) |
| Week 2 | 1000 mcg | 20 units (0.20 mL) |
| Week 3 | 1500 mcg | 30 units (0.30 mL) |
| Week 4 | 2000 mcg | 40 units (0.40 mL) |
| Week 5 | 2500 mcg | 50 units (0.50 mL) |
| Week 6 | 3000 mcg | 60 units (0.60 mL) |
| Week 7 | 3500 mcg | 70 units (0.70 mL) |
| Week 8 | 4000 mcg | 80 units (0.80 mL) |
| Week 9 | 4500 mcg | 90 units (0.90 mL) |
| Weeks 10–16 | 5000 mcg | 100 units (1.00 mL) |
For ≤10-unit (≤0.10 mL) administrations (Week 1), consider 30- or 50-unit insulin syringes for improved readability.
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to avoid foaming.
- Gently swirl or roll until fully dissolved (do not shake vigorously).
- Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
- Use within 2–4 weeks of reconstitution for optimal potency.
