Description
Glutathione (GSH) is a tripeptide composed of glutamine, cysteine, and glycine that serves as the body’s master antioxidant. High‑purity reduced L‑glutathione (≥99%) is supplied in 1500mg vials for research on oxidative stress reduction, detoxification support, and cellular protection. This educational protocol presents a subcutaneous (SC) injection approach using moderate weekly dosing with clear reconstitution guidance.
- Reconstitute: Add 6.0 mL bacteriostatic water → 250 mg/mL concentration.
- Typical range: 100–200 mg per injection, 2–3 times weekly (300–600 mg/week total).
- Easy measuring: At 200 mg/mL, 1 unit = 0.01 mL = 2.5mg (2,000 mcg) on a U‑100 insulin syringe.
- Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F); do not freeze reconstituted solution.
Standard / Gradual Approach (3 mL = 200 mg/mL)
| Week / Phase | Per‑Injection Dose | Units (mL) |
|---|---|---|
| Weeks 1–2 (Initiation) | 100 mg (100,000 mcg) | 40 units (0.50 mL) |
| Weeks 3–8 (Maintenance) | 200 mg (200,000 mcg) | 80 units (1.0 mL) |
| Weeks 9–12 (Extended) | 200 mg (200,000 mcg) | 80 units (1.0 mL) |
Frequency: Inject 3 times weekly (e.g., Monday/Wednesday/Friday) subcutaneously. This yields 300 mg/week during initiation and 600 mg/week during maintenance phases. Common research protocols use 100–200 mg per injection, 2–3×/week for antioxidant support.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to minimize foaming; glutathione solutions should remain clear to pale yellow.
- Gently swirl or roll until fully dissolved (do not shake vigorously).
- Label with reconstitution date and refrigerate immediately at 2–8 °C (35.6–46.4 °F), protected from light.
- Do not freeze the reconstituted solution; freezing may cause precipitation.
